Anti-Human Cytokeratin 6/18 – Purified,C161,Leinco
Clone: LP34
Product Type: Monoclonal Antibody
Isotype: Mouse IgG1 κ
Applications: IHC (Frozen), IHC (Paraffin)
|
Prod No.
|
Size
|
Price
|
Avail.
|
Qty
|
Add to cart
|
|
|
|
|
|
|
|
Antibody Details
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage ?
(Quality Tested by Leinco)
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 15 μg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Immunohistochemistry on Frozen Sections: This antibody should give satisfactory staining results when used on frozen sections at a concentration of 10 μg/ml.
Description
Specificity
Mouse Anti-Human Cytokeratin 18,6 (Clone LP34) recognizes Human Cytokeratin 18,6. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Anti-Human Cytokeratin 18, 6 (clone LP34) reacts with the cytokeratin polypeptides 18 and 6, with molecular weights of 45 kDa and 56 kDa, respectively. The antibody does not react with keratins 1, 8, or 19. Anti-Cytokeratin 18, 6 reacts in cryostat sections with epithelial tissues known to contain filamentous keratins. It reacts with single glandular epithelia to stratified squamous epithelia. Anti-Cytokeratin 18,6 may be used in research studies to identify tumors as being of epithelial or mesothelial origin enabling aplastic carcinomas to be distinguished from other non-carcinoma types of tumors.1,2 The antibody may also be used to trace areas of epithelial tissue in mixed tumors.
Antigen Details
References & Citations
1. Moll, WR. et al. (1982) Cell 31:11
2. Gatter, KC. et al. (1982) J. Clin. Path. 35:125
Technical Protocols
