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This fluorescein conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.

This Fluorescein conjugate is stable when stored at 2-8°C. Do not freeze.

Flow Cytometry: This antibody has been quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 μg per million cells in 100 μl volume. Each investigator should determine their own optimal working dilution for specific applications.

Western Blotting: Bit1 antibody can be used for the detection of Bit1 by Western blot at 1 - 4 μg/mL.

Flow Cytometry: This antibody can be used at a concentration of ≤ 0.25 μg per 100 μl of whole blood (or per 1.0 x 10⁶ cells in a 100 μl total staining volume).

Flow Cytometry: 1234

Immunocytochemistry: 1234

Blue Laser (488 nm)

Anti-Human HLA-A, B, C Monomorphic (Clone W6/32) recognizes a monomorphic determinate (α chain) of major histocompatibility HLA Class I antigens. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

This HLA-A, B, C antigen is present on most nucleated cells of humans and old world monkeys.

CD3/TCR, CD8

1. Yang, Yong-Guang et al. (2006) Blood 108 (2): 487-492. 
2. Barnstable, C. et al. (1978) Cell 16:9
3. Brodsky, F. et al. (1979) Immunol. Rev. 47:3