Use the iScript cDNA Synthesis Kit for first-strand cDNA synthesis for real-time qPCR. This two-tube kit can accommodate dilute RNA samples.
Key Features and Benefits
· Up to 15 μl of RNA in a 20 μl cDNA synthesis reaction
· Combined oligo(dT) and random primers provide unbiased representation of 5' and 3' regions
· Fast 26 min cDNA synthesis protocol
· Linear range of 1 μg–1 pg total input RNA using RNase H+ MMLV reverse transcriptase
Packaging Options
Number of 20 μl Reactions |
Supermix Volume, μl |
Catalog Number |
25 |
100 (1 x 100 μl) |
1708890 |
100 |
400 (1 x 400 μl) |
1708891 |
500 |
2,000 (5 x 400 μl) |
1708891BUN |
See the iScript cDNA Synthesis Kit product page for more information and documentation.
Related Products
· View all cDNA Synthesis Kits
· Real-Time PCR Supermixes and Kits
· PrimePCR? PCR Primers & Assays
Related Categories
· PCR and qPCR Reagents
· Real-Time PCR Detection Systems
· PCR Plastic Consumables
· Instrument Validation Tools and Services
Supporting Documents
· iScript cDNA Synthesis Kit Product Insert
· Amplification Plastics and Reagents Selection Guide
Online Resources
· qPCR/Real-Time PCR
· Genomics