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rMuDKK-1; mDkk-1; Dickkopf-1 ; 重組小鼠 DKK-1 (CHO細胞表達)

Mouse

CHO

O54908

13380

19-20 kDa

SATLNSVLIN SNAIKNLPPP LGGAGGQPGS AVSVAPGVLY EGGNKYQTLD NYQPYPCAED EECGSDEYCS SPSRGAAGVG GVQICLACRK RRKRCMRHAM CCPGNYCKNG ICMPSDHSHF PRGEIEESII ENLGNDHNAA AGDGYPRRTT LTSKIYHTKG QEGSVCLRSS DCAAGLCCAR HFWSKICKPV LKEGQVCTKH KRKGSHGLEI FQRCYCGEGL ACRIQKDHHQ AS

The ED₅₀ is <6 μg/mL as measured in stimulation of alkaline phosphatase activity using CCl-226 cells.

Lyophilized powder.

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by LAL method.

Reconstitute the lyophilized recombinant Mouse DKK-1 (CHO-expressed) (rMuDKK-1) to 100 μg/mL using ddH₂O or PBS.

Lyophilized recombinant Mouse DKK-1 (CHO-expressed) (rMuDKK-1) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

Mature mouse Dkk-1 is a 40 kDa glycosylated protein that shares 86%, 96%, 83% and 82% amino acid (aa) sequence identity with human, rat, rabbit and bovine Dkk?1, respectively. It also shares 41% and 36% aa identity with human Dkk-2 and Dkk-4, respectively^[1]. Dkk1 is a secreted Wnt inhibitor and member of a distinct multigene family, this inhibition plays a key role in heart, head and forelimb development during anterior morphogenesis of the embryo.