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Human/Mouse/Rat SOX2 Antibody

Human/Mouse/Rat SOX2 Antibody

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商品描述

Human/Mouse/Rat SOX2 Antibody，MAB2018,R&D：

Human/Mouse/Rat SOX2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat SOX2 in Western blots.
Source
Monoclonal Mouse IgG2A Clone # 245610
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human SOX2
Gly135-Met317
Accession # P48431
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 μg/mL
See below
Simple Western
4 μg/mL
See below
CyTOF-reported
Zunder, E.R. et al. (2015) Cell Stem Cell 16: 323. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 μg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 μg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry E?Cadherin and SOX2 in BG01V Human Stem Cells. View Larger

E?Cadherin and SOX2 in BG01V Human Stem Cells. E?Cadherin and SOX2 were detected in BG01V human embryonic stem cells using 10 μg/mL Goat Anti-Human E?Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF648) and 10 μg/mL Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for E?Cadherin using the NorthernLights? 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and for SOX2 using the NorthernLights 493-conjugated Anti-Mouse Secondary Antibody (green; Catalog # NL009). Cells were counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western Blot Detection of Human, Mouse, and Rat SOX2 by Western Blot. View Larger

Detection of Human, Mouse, and Rat SOX2 by Western Blot. Western blot shows lysates of NTera?2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, D3 mouse embryonic stem cell line, and rat cortical stem cells. PVDF membrane was probed with 1 μg/mL of Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SOX2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry SOX2 in BG01V Human Embryonic Stem Cells. View Larger

SOX2 in BG01V Human Embryonic Stem Cells. SOX2 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human/
Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018) at 10 μg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights? 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of SOX2 in NTera?2 Human Cell Line by Flow Cytometry. View Larger

Detection of SOX2 in NTera?2 Human Cell Line by Flow Cytometry. NTera?2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/
Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).  View our protocol for Staining Intracellular Molecules.

Simple Western Detection of Human SOX2 by Simple WesternTM. View Larger

Detection of Human SOX2 by Simple WesternTM. Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for SOX2 at approximately 50 kDa (as indicated) using 4 μg/mL of Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.


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