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Human SCF

Human SCF

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商品描述
 Human SCF ，130-096-695，MiltenyiBiotec:
overview：
SCF stands for stem cell factor, also termed c-kit ligand, mast cell growth factor (MGF), or steel factor (SLF). Human SCF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human SCF can be used for a variety of applications, including:
  • Stimulation of proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures.
  • In vitro expansion of CD34+ hematopoietic progenitor cells.
  • Differentiation of ES-derived cells towards the hematopoietic lineage.
  • Mast cell differentiation and maintenance.
  • Mast cell chemotaxis assays.

Alternative names

c-kit ligand, steel factor, MGF

Details:

Background information

SCF is a hematopoietic growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and progenitor cells. Besides its pivotal role in mast cell development, SCF acts as a potent mast cell chemoattractant and upregulates mast cell adhesion and migration. SCF signals through the c-kit receptor (CD117) and exists in two forms; cell surface bound SCF and soluble SCF. The secreted soluble form of SCF is produced by the proteolytic processing of the cell surface anchored precursor molecule.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of TF-1 cells (NIBSC 91/682)
  • premium grade: ≥ 4×
    10
    5
    U/mg
    (typical activity: ≥ 9×
    10
    5
    U/mg
    )
  • research grade: ≥ 2×
    10
    5
    U/mg
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