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Product name

Anti-CD41 antibody [M148]
See all CD41 primary antibodies

Description

Mouse monoclonal [M148] to CD41

Host species

Mouse

Specificity

ab11024 binds to some medulloblastoma and neuroblastoma cells and rhabdomyosarcoma and certain other solid tumours. No binding observed to marrow constituents other than platelets and megakaryocytes.

Tested applications

Suitable for: Flow Cyt,ICC/IF,IP,IHC-P,IHC-Fr

Species reactivity

Reacts with: Human

Immunogen

Tissue, cells or virus corresponding to Human CD41. Homogenized human medulloblastoma tissue.
Database link: P08514

Form

Liquid

Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

Storage buffer

Constituent: PBS

Purity

Protein A purified

Clonality

Monoclonal

Clone number

M148

Myeloma

P3x63-Ag8.653

Isotype

IgG1

IHC-Fr image of C41 staining on human blood film using ab11024 (1:1000). The sample was fixed using paraformaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The slides were then blocked with 10% Donkey Serum for 1 hr at 24°C. ab11024 was diluted 1:100 using 0.1% TritonX with 0.1x PBS- 10% Donkeys and slides were incubated with the primary antibody for 4 hours at 24°C. The secondary antibody used was Donkey Polyclonal to rabbit IgG conjugated to Alexa Fuor 568 (1:1000)

ICC/IF image of ab11024 staining of human platelet cells. The sections were incubated in 10% serum to block non-specific protein-protein interactions and in 0.2% triton X to permeabilise the cells. The sections were then incubated with ab11024 (1:400) for one hour at 20°C, followed by alexa 488 conjugated secondary antibody (green). Platelets were counterstained with rhodamine phalloidin to outline the cellular cytoskeleton (red).

Overlay histogram showing HL60 cells stained with ab11024 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11024, 1μg/1x10⁶ cells) for 30 min at 22oC. The secondary antibody used was DyLight? 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22oC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed HL60 cells used under the same conditions.

Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.