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Product name

Anti-beta Amyloid antibody [DE2B4]
See all beta Amyloid primary antibodies

Description

Mouse monoclonal [DE2B4] to beta Amyloid

Host species

Mouse

Tested applications

Suitable for: ICC/IF,IHC-Fr,WB,IHC-P,IP

Species reactivity

Reacts with: Human

Immunogen

Synthetic peptide, corresponding to amino acids 1-17 of Human beta Amyloid.

Epitope

ab11132 recognizes an epitope in the region 8-16 of the amyloid precursor protein.

Positive control

• Alzheimer's disease brain.

General notes

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Form

Liquid

Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Storage buffer

Preservative: 0.09% Sodium azide
Constituent: PBS

Concentration information loading...

Purity

Protein G purified

Purification notes

Purified from tissue culture supernatant.

Clonality

Monoclonal

Clone number

DE2B4

Isotype

IgG1

ab11132 at 1/100 staining human brain (Alzheimer's diseased) tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed prior to incubation with ab11132 for 16 hours. A biotinylated goat anti-mouse antibody was used as the secondary.

In this experimental set up ab11132 shows clear extracellular and intracellular staining.

Immunofluorescence detection using Mouse monoclonal [DE2B4] to beta Amyloid (ab11132) on Human HEK cells. Cells were fixed using Methanol, no antigen retrieval step was necessary. Primary antibody ab11132 was incubated for 1hr at room temperature (1/400). The confocal image of transfected HEK cells shows A. anti-amyloid beta (ab11132), B. swAPP-GFP, C. Overlay. Significant colocalisation is seen as would be expected. (GFP is tagged to C-term of swAPP).

ab11132 staining beta amyloid in 15 month TASTPM mouse brain tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, antigen retrieval in 70% Formic acid buffer for 20 mins and blocking in 1% BSA for 10 mintes at 25°C. The primary antibody was used at 1/100 dilution and incubated with sample for 2 hours. A Biotin conjugated goat polyclonal to mouse IgG1 at 1/200 dilution, was used as secondary.

Immunohistochemistical detection of beta Amyloid using antibody [DE2B4] (ab11132) on transgenic APP23 mouse brain cortex frozen sections. Fixative: Formaldehyde Permeabilization: Yes - 0.1% Tween 20 Blocking step: 1% BSA for 30 mins @ 1 % @ rt°C. Primary Antibody used at 1/200 and incubated for 2 hours in TBS/BSA/Tween 20/azide. Secondary antibody: anti Mouse IgG Alexa Fluor? 594 (1/1000).  Immunstaining performed on APP overexpressing TG mouse (APP23) tissue. Unfixed cryosections were fixed in 4% Formalin in PBS before treatment with 70% Formic acid to most effectively reveal antigenic sites. Interestingly, punctate intracytoplasmic positivity is also observed in neurones, using this Ab: this may be APP. Note the fibrillar positivity within the plaque: also that is has a negative centre (older plaques seem to calcify and this is often dislodged).

CHO-K1 cells, expressing either wild-type or Swedish mutant APP, were transfected with the vector encoding stefin B and lysed after 24 hours. The lysates were immunoprecipitated with a polyclonal anti-rabbit stefin B antibody, after which 15% SDS gels and Western blotting with ab11132 at a 1/400 dilution were performed. First and second lanes show results obtained on whole cell lysates of APP-WT and APP-Sw, respectively, and the third and fourth lanes show the corresponding samples after co-immunoprecipitation on anti-stefin B antibody. ab11132 clearly detects a fragment of 15 kDa (according to molecular mass standards) in the APP-WT cells.
For further details please see reference.