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Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)

Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)

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商品描述
  • Product name
    Anti-alpha smooth muscle Actin antibody [4A4]
    See all alpha smooth muscle Actin primary antibodies
  • Description
    Mouse monoclonal [4A4] to alpha smooth muscle Actin
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB,ELISA,IHC-P,Flow Cyt,ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Monkey
  • Immunogen

    Synthetic peptide of Human alpha smooth muscle Actin (C-EYDEAGPSIVHRKCF) conjugated KLH

  • Positive control
    • Recombinant alpha smooth muscle Actin protein, Hela, A431, Jurkat, K562, HEK293, HepG2, NIH3T3, PC12 and COS7 cell lysates, HepG2 cells, HeLa cells
  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration
    • 100 μl at 1 mg/ml

  • Purity
    Protein G purified
  • Purification notes
    Purified from tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    4A4
  • Isotype
    IgG1

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)
    Immunohistochemical analysis of paraffin-embedded Human duodenum tissue using ab119952 at a dilution of 1/200 with DAB staining.

  • Western blot - Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)
    Western blot - Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)
    All lanes : Anti-alpha smooth muscle Actin antibody [4A4] (ab119952) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : K562 cell lysate
    Lane 5 : HEK293 cell lysate
    Lane 6 : HepG2 cell lysate
    Lane 7 : NIH3T3 cell lysate
    Lane 8 : PC12 cell lysate
    Lane 9 : COS7 cell lysate

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)
    Immunohistochemical analysis of paraffin-embedded Human esophagus tissue using ab119952 at a dilution of 1/200 with DAB staining.

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)
    Immunofluorescence analysis of HepG2 cells using ab119952 at 1/200 (green). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (Red). DRAQ5 fluorescent DNA dye (blue).

  • Flow Cytometry - Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)
    Flow Cytometry - Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)
    Flow cytometric analysis of Hela cells using ab119952 at a dilution of 1/200 (green) and negative control (purple).

  • ELISA - Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)
    ELISA - Anti-alpha smooth muscle Actin antibody [4A4] (ab119952)
    ELISA, using ab119952 at a dilution of 1/10000.




Compatible Secondaries
　
Goat Anti-Mouse IgG H&L (Alexa Fluor? 488) (ab150113) 
Goat Anti-Mouse IgG H&L (HRP) (ab205719) 
Immunohistochemistry kits
　
Mouse on Mouse Polymer IHC Kit (ab127055) 
Isotype control
　
Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190) 
Positive Controls
　
HepG2 cytoplasmic extract lysate (ab14659) 
Jurkat cytoplasmic extract lysate (ab14842) 
NIH 3T3 cytoplasmic extract lysate (ab14873) 
PC12 cytoplasmic extract lysate (ab14883) 
Recombinant Protein
　
Recombinant Human alpha smooth muscle Actin protein (ab114148) 
Recombinant Human alpha smooth muscle Actin protein (ab134555) 
Related Products
　
Human alpha smooth muscle Actin peptide (ab16102) 
Phalloidin-iFluor 488 Reagent (ab176753) 
Phalloidin-iFluor 647 Reagent (ab176759)