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人白介素15(IL-15)ELISA Kit CSB-E04603h

人白介素15(IL-15)ELISA Kit CSB-E04603h

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商品描述


  • 產品類型:
    ELISA Kit
  • 產品名稱:

    人白介素15(IL-15)ELISA Kit

  • 英文名稱:

    Human Interleukin 15,IL-15 ELISA KIT

  • 貨號:
    CSB-E04603h
  • 別名:
    IL-15, MGC9721
  • 規(guī)格:
    96T
  • 中文價格:
    3600
  • 種屬:
    Homo sapiens (Human)
  • 待測物名稱:
    interleukin 15
  • 縮寫:
    IL15
  • 檢測范圍:
    3.12 pg/mL-200 pg/mL
  • 靈敏度:
    0.78 pg/mL
  • 反應時間:
    1-5h
  • 所需樣本體積:
    50-100ul
  • 檢測波長:
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    200 2.536 2.436 2.486 2.345  
    100 2.001 1.901 1.951 1.810  
    50 1.308 1.208 1.258 1.117  
    25 0.815 0.805 0.810 0.669  
    12.5 0.453 0.443 0.448 0.307  
    6.25 0.324 0.314 0.319 0.178  
    3.12 0.231 0.222 0.227 0.086  
    0 0.141 0.140 0.141  
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    Sample Serum(n=4)  
    1:1 Average % 84  
    Range % 80-93  
    1:2 Average % 94  
    Range % 91-99  
    1:4 Average % 90  
    Range % 85-96  
    1:8 Average % 96  
  • 回收率:
    The recovery of human IL-15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 89-99  
    EDTA plasma (n=4) 89 86-93  
                 
                 
  • 定性/定量:
    quantitative
  • 研究領域:
    Immunology